Minoxidil Response Test
Introducing the World’s first Rapid Test to check Minoxidil response in 60 minutes.
Now no need to wait for longer periods to know minoxidil efficacy for Androgenic Alopecia treatment.
“Know Your Minoxidil Response in Less Than 60 Minutes!”
Mechanism behind Rapid Minoxidil Response Test
Rapid Minoxidil Response Test is a biochemical assay based on plucked hair follicle sulfation reaction
Principle:
The plucked hair follicle sulfation reaction involves the transfer of a sulfonyl group (SO3-) from a donor molecule, 3′-phosphoadenosine-5′-phosphosulfate (PAPS), to an acceptor molecule, p-nitrophenol (PNP), catalyzed by the enzyme sulfotransferase. This reaction can be represented as follows:
PAPS + PNP → PAP + p-nitrophenylsulfate (PNPS)
The sulfonyl group is a highly reactive group that can be transferred to a variety of acceptor molecules, including phenolic compounds like PNP. The reaction is driven by the hydrolysis of the high-energy phosphate bond in PAPS, which releases energy that is used to overcome the activation barrier for the sulfonyl group transfer.
Enzyme-Substrate Mechanism
The enzyme sulfotransferase plays a crucial role in facilitating the sulfation reaction by bringing the donor and acceptor molecules together and aligning their binding sites. The enzyme has two distinct binding sites: one for PAPS and one for the acceptor molecule. When PAPS and PNP bind to their respective sites, the enzyme undergoes a conformational change that brings the sulfonyl group of PAPS into close proximity to the hydroxyl group of PNP. This alignment facilitates the nucleophilic attack of the hydroxyl group on the sulfonyl group, leading to the formation of PNPS and the release of PAP.
The enzyme-substrate mechanism of the sulfation reaction can be summarized as follows:
1. Binding: PAPS and PNP bind to their respective binding sites on the enzyme.
2. Conformational Change: The enzyme undergoes a conformational change, bringing the sulfonyl group of PAPS and the hydroxyl group of PNP into close proximity.
3. Nucleophilic Attack: The hydroxyl group of PNP attacks the sulfonyl group of PAPS, forming a covalent bond and releasing pyrophosphate.
4. Product Release: The enzyme releases PNPS and regenerates its active form, ready for another catalytic cycle.
Why wait? Within 60 minutes discover if Minoxidil will work for you.
Take the Minoxidil Response Test today.
Key Features:
Rapid Testing: Get results in under 60 minutes, allowing for immediate decision-making
Instant Feedback: Receive instant feedback on your body’s response to minoxidil, empowering you to make informed choices.
Convenient Process: Quick and hassle-free testing done from the comfort of your premises.
Expert Consultation: Access to professional guidance and personalized recommendations based on your test results.
How It Works?
It is a simple qualitative assay:
- Collect 2 hair strands with a bulb.
- Place them carefully in a provided collection tube containing the substrate chemical mixture.
- Incubate for 60 minutes at room temperature.
- Based on the color change, we can determine whether a person responds to Minoxidil treatment.